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Use Aseptic Technique: A sterile syringe and needle must be used for every injection to prevent contamination of the multi-dose vial between uses

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"We're seeing clear changes in the muscles in these patients," said study lead author Michle van Vugt, a professor of internal medicine at Amsterdam University Medical Center (UMC)

doi:10.1371/journal.pone.0033944 Lederman RJ, Mendelsohn FO, Anderson RD, Saucedo JF, Tenaglia AN, Hermiller JB, Hillegass WB, Rocha-Singh K, Moon TE, Whitehouse MJ et al (2002) Therapeutic angiogenesis with recombinant fibroblast growth factor-2 for intermittent claudication (the TRAFFIC study): a randomised trial